Intracellular pH-determination by fluorescence measurements.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fluorescence Lifetime pH Measurements in Cheese Matrix

The pH of cheese affects the texture of curd directly by influencing the hydratation of the caseins; all else being equal, high pH cheeses are softer than more acid cheeses. pH also affects texture and flavour indirectly by affecting the activity of enzymes important to ripening and, in the case of the coagulant, the retention of enzyme in the curd during manufacture [1]. Since low pH is known ...

متن کامل

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

متن کامل

Raman/fluorescence dual-sensing and imaging of intracellular pH distribution.

Herein, a pH-sensitive probe has been designed based on DNA modified gold nanoparticles (AuNPs) for Raman/fluorescence dual-imaging of the intracellular pH distribution. In the acidic environment of cancer cells, the configuration change of DNA on the probe surface could turn "on" Raman/fluorescence signals simultaneously.

متن کامل

Quantitative fluorescence measures for determination of intracellular perforin content.

We present methodologic details and operating characteristics of a procedure with whole blood for the quantitative assessment of intracellular perforin within distinct lymphocyte subsets. Using this method, we analyzed 20 healthy controls and 2 individuals with an inherited deficiency of perforin. The mean +/- standard deviation perforin contents of natural killer (NK) cells and cytotoxic T cel...

متن کامل

Measurements of the Intracellular Ph in a Single Cell of Nitella Flexilis by Means of Micro-glass Ph Electrodes.

The use of micro-glass electrodes sensitive to the activity of selected cations has permitted a direct measurement of intracellular ionic activity , e. g., the activity of hydrogen ion in a single crab muscle fiber (CALDWELL 1954), and in a single squid giant axon (CALDWELL 1958, SPYROPOULOS 1960), the activity of sodium and potassium ion in a single squid giant axon (HINKE 1960). With a micro-...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Histochemistry & Cytochemistry

سال: 1979

ISSN: 0022-1554,1551-5044

DOI: 10.1177/27.1.35567